摘要

据报道，医院废水（HWWs）是抗生素和耐药菌的研究热点。然而，关于这些废水对抗生素耐药基因（ARGs）传播的影响的研究较少。因此，在本研究中，采用定量聚合酶链反应方法监测季节性收集的HWWs的细菌总数和7个ARGs aadA、tetA、cmlA、sul1、qnrS、ermB和bla CTX-M。细菌16sRNA拷贝数冬季最低，为103拷贝/mL；夏季和春季最高，为105拷贝/mL。所有被检测的医院都显示了类似的季节性ARGs拷贝数分布，即aadA > tetA > cmlA sul1 > ermB qnrS > bla CTX-M。结果表明，未经处理医院废水是ARGs的热点，在排入公共下水道前需要注意。

Hospital wastewaters (HWWs) are reported to be hotspots for antibiotics and antibiotic-resistant bacteria. However, limited information involves the impact of these effluents on dissemination of antibiotic-resistance genes (ARGs). In this study, therefore, seasonally collected HWWs were monitored for overall bacterial load and seven ARGs aadA, tetA, cmlA, sul1, qnrS, ermB and bla _CTX-M by using quantitative polymerase chain reaction method. Overall bacterial 16S rRNA copy number was found to be the lowest in winter with 10³ copy number/mL, while the highest copy number, with 10⁵ copy number/mL, was observed in both summer and spring. All hospitals tested displayed similar seasonal ARG copy number profile of aadA > tetA > cmlA ≈ sul1 > ermB ≈ qnrS > bla _CTX-M. The results indicated that untreated HWWs were hotspots for ARGs and required attention before discharging into public sewer.

https://link.springer.com/article/10.1007/s00128-020-02792-2