摘要

       研究了氧化石墨烯（GO）对猪粪厌氧消化（AD）性能、微生物群落和抗生素抗性基因（ARGs）还原的影响。结果表明，GO浓度为5mg/L、50mg/L、100mg/L和500mg/L时，甲烷的生成量分别降低了13.1%、10.6%、2.7%和17.1%，但随着GO的加入，丙酸的降解作用增强。添加GO后，细菌和古细菌群落变化不大。AD能明显降低ARGs的丰度，但在GO浓度为50mg/L和100mg/L时，其丰度有所下降，在500mg/L时，其丰度有所增加，而在5mg/L时则无明显变化。网络分析和扫描电镜分析表明，各ARG的变化与微生物群落组成的变化密切相关，环境变量对ARGs丰度的影响最大结果表明，GO对ARGs的动态有负向影响，其中以重金属抗性基因（MRGs）的影响最为直接。

         The role of graphene oxide (GO) on anaerobic digestion (AD) of swine manure concerning the performance, microbial community and antibiotic resistance genes (ARGs) reduction was investigated. Results showed that methane production was reduced by 13.1%, 10.6%, 2.7% and 17.1% at GO concentration of 5mg/L, 50mg/L, 100mg/L and 500mg/L, respectively, but propionate degradation was enhanced along with GO addition. Both bacterial and archaeal community changed little after GO addition. AD could well reduce ARGs abundance, but it was deteriorated at the GO concentration of 50mg/L and 100mg/L and enhanced at 500mg/L, while no obvious changes at 5mg/L. Network and SEM analysis indicated that changes of each ARG was closely associated with variation of microbial community composition, environmental variables contributed most to the dynamics of ARGs indirectly, GO influenced the ARGs dynamics negatively and (heavy metal resistance genes (MRGs)) influenced the most directly.

          https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(17)31531-6