摘要

        转基因棉花生产产生的秸秆产量大，处理难度大。为了研究转基因棉秆堆肥的可行性和堆肥过程中抗生素抗性基因（ARGs）的变化，我们在猪粪中添加了转基因或非转基因棉秆，比较了两种处理对intI1、intI2和ARGs绝对丰度（AA）的影响。根据发芽指数和C/N比，堆肥经处理后成熟。堆肥后，在非转基因和转基因处理下，ARGs、intI1和intI2的AAs分别降低了41.7%和45.0%。温度和氨氮对ARG谱有显著影响。除tetC外，转基因棉秆对ARGs、intI1和intI2的影响与非转基因处理相比无显著性差异（p<0.05）。因此，与非转基因棉秆类似，转基因棉秆可用于畜禽粪便的好氧堆肥，降低了ARGs的AAs。研究结果为转基因棉秆的无害化利用提供了理论依据。

         Genetically modified (GM) cotton production generates a large yield of stalks and their disposal is difficult. In order to study the feasibility of using GM cotton stalks for composting and the changes that occur in antibiotic resistance genes (ARGs) during composting, we supplemented pig manure with GM or non-GM cotton stalks during composting and we compared their effects on the absolute abundances (AA) of intI1, intI2, and ARGs under the two treatments. The compost was mature after processing based on the germination index and C/N ratio. After composting, the AAs of ARGs, intI1, and intI2 were reduced by 41.7% and 45.0% in the non-GM and GM treatments, respectively. The ARG profiles were affected significantly by temperature and ammonia nitrogen. In addition, excluding tetC, GM cotton stalks had no significant effects on ARGs, intI1, and intI2 compared with the non-GM treatment (p < 0.05). Thus, similar to non-GM cotton stalks, GM cotton stalks can be used for aerobic composting with livestock manure, and the AAs of ARGs can be reduced. Furthermore, the results of this study provide a theoretical basis for the harmless utilization of GM cotton stalks.

          https://www.sciencedirect.com/science/article/abs/pii/S0147651317305924?via%3Dihub