摘要

       城市河流中的抗生素耐药基因（ARGs）在规划落后、发展迅速的地区是一个严重的公共卫生问题。为深入了解我国东部一条高污染城市河流中影响ARGs归宿的主要因素，对17个地点的285个ARGs、微生物群落和20个理化参数进行了分析。高通量qPCR共检测到258个独特的ARGs，总ARGs的绝对丰度与总有机碳和总溶解氮浓度呈正相关（P<0.01）。微生物群落结构改变了ARG的丰度和多样性。变异分配分析表明，多种因素的综合作用有助于ARGs的分布和传播，微生物群落的变异是影响ARGs分布的主要因素。哺乳动物肠道菌群中的类杆菌、人畜共患传染病中的伯克霍尔德菌和污水处理中的动物胶菌等细菌的分布差异表明，城市河流受点源污染的影响较大。结果表明，水质变化引起的微生物群落变化可能导致ARGs的扩散，城市河流点源污染对控制ARGs在环境细菌和病原菌之间的迁移具有重要意义。

        Antibiotic resistance genes (ARGs) in urban rivers are a serious public health concern in regions with poorly planned, rapid development. To gain insights into the predominant factors affecting the fate of ARGs in a highly polluted urban river in eastern China, a total of 285 ARGs, microbial communities, and 20 physicochemical parameters were analyzed for 17 sites. A total of 258 unique ARGs were detected using high-throughput qPCR, and the absolute abundance of total ARGs was positively correlated with total organic carbon and total dissolved nitrogen concentrations (P < 0.01). ARG abundance and diversity were greatly altered by microbial community structure. Variation partitioning analysis showed that the combined effects of multiple factors contributed to the profile and dissemination of ARGs, and variation of microbial communities was the major factor affecting the distribution of ARGs. The disparate distribution of some bacteria, including Bacteroides from mammalian gastrointestinal flora, Burkholderia from zoonotic infectious diseases, and Zoogloea from wastewater treatment, indicates that the urban river was strongly influenced by point-source pollution. Results imply that microbial community shifts caused by changes in water quality may lead to the spread of ARGs, and point-source pollution in urban rivers requires greater attention to control the transfer of ARGs between environmental bacteria and pathogens.

        https://escholarship.org/uc/item/48r9n2vj