摘要

       抗生素耐药菌（ARB）和抗生素耐药基因（ARGs）在各种环境基质中的普遍存在，对人类和生态健康构成潜在威胁。因此，采用光电催化（PEC）技术研究了ARB大肠杆菌S1-23的灭活及其相关的arg（blaTEM-1和aac（3）-II）的消除。结果表明，ARB大肠杆菌S1-23（1×108cfu-mL-1）及其ARGs（胞外和胞内）在PEC处理10和16h内均能完全失活。相比之下，光催化（PC）和电化学（EC）处理没有明显的效果，但从大肠杆菌S1-23中提取的含精氨酸的DNA可以在几分钟内被完全分解。进一步的PCR、AFM和HPLC分析表明，裸ARGs的结构完整性和表面形貌在处理过程中受到破坏，可以完全消除。此外，在PEC、PC和EC治疗期间，没有产生胞嘧啶、鸟嘌呤、腺嘌呤或胸腺嘧啶中间体。本研究首次提出PEC处理是一种很有前途的完全分解ARB和ARGs的方法。

        The ubiquity of antibiotic-resistance bacteria (ARB) and antibiotic-resistance genes (ARGs) in various environmental matrices is a potential threat to human and ecological health. Therefore, the inactivation of ARB E. coli S1-23 and the elimination of its associated ARGs, bla_TEM-1 and aac(3)-II, were investigated using the photoelectrocatalytic (PEC) process. Results indicate that the ARB E. coli S1-23 (1 × 10⁸ cfu mL^-1) and its ARGs (extracellular and intracellular) could be fully inactivated within 10 and 16 h PEC treatment, respectively. In contrast, photocatalytic (PC) and electrochemical (EC) treatments displayed no obvious effect; however, ARG-containing DNA extracted from E. coli S1-23, which was used as a model for dissociative naked ARGs, could be completely decomposed within a few minutes through these three treatments. Further analyses, including PCR, AFM and HPLC, proved that the structural integrity and surface topography of naked ARGs are damaged during treatment and can be completely eliminated. Furthermore, there is no generation of cytosine, guanine, adenine or thymine intermediates during the PEC, PC, and EC treatments. This study is the first report to propose the PEC treatment as a promising method for complete decomposition of ARB and ARGs in aqueous systems.

        https://www.sciencedirect.com/science/article/abs/pii/S0043135417307121?via%3Dihub