摘要

厌氧膜生物反应器（AnMBRs）是一种新兴的污水处理技术，具有提高能源利用率和污水回用的潜力。然而，它们对抗生素耐药基因（ARGs）等新出现的污染物的扩散所起的作用，目前还不清楚。本研究的目的是探讨不同浓度的抗生素对AnMBR系统及其出水中ARGs存在和丰度的影响。靶向ARGs的定量显示了生物量和废水中的不同特征，在生物量（大环内酯类）和废水（磺胺类）中，具有不同抗生素类抗性的基因占主导地位。出水sul1基因丰度与intl1基因丰度密切相关，表明流动基因在AnMBR系统ARG释放中的潜在重要性。特定抗生素的添加也影响其相关arg的标准化丰度，说明在低（10μg/L）和高（250μg/L）的进水抗生素浓度下选择性压力的潜在影响。

        Anaerobic membrane bioreactors (AnMBRs) are an emerging technology with potential to improve energy efficiency and effluent reuse in mainstream wastewater treatment. However, their contribution to the proliferation of contaminants of emerging concern, such as antibiotic resistance genes (ARGs), remains largely unknown. The purpose of this study was to determine the effect of select influent antibiotics at varying concentrations on the presence and abundance of ARGs in an AnMBR system and its effluent. Quantification of targeted ARGs revealed distinct profiles in biomass and effluent, with genes conferring resistance to different antibiotic classes dominating in biomass (macrolides) and effluent (sulfonamides). Effluent sul1 gene abundance was strongly correlated with abundance of intl1, signifying the potential importance of mobile genetic elements in ARG release from AnMBR systems. The addition of specific antibiotics also affected normalized abundances of their related ARGs, exemplifying the potential impact of selective pressures at both low (10 μg/L) and high (250 μg/L) influent antibiotic concentrations.

        https://pubs.acs.org/doi/10.1021/acs.est.9b00798