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“农业废弃物至宝”-生物炭和蛋壳阻止土壤抗生素/抗生素抗性细菌(基因)在马铃薯中积累

发布者:抗性基因网 时间:2020-04-16 浏览量:995

      摘要

      土壤中抗生素和抗生素耐药细菌/基因(ARB/ARGs)的污染已成为一个新出现的环境问题。此外,混合污染物从土壤向块茎类蔬菜的转移和积累对粮食安全和人类健康构成了巨大威胁。本研究采用玉米生物炭和硫酸盐改性蛋壳两种吸附材料,通过促进水溶性抗生素在土壤中的消散,降低土壤抗生素对马铃薯根系的毒害作用,并通过增加马铃薯淀粉、蛋白质、脂肪和维生素,改善食品品质。同时,两种改良剂均能有效降低马铃薯可食部分ARB和ARGs(sulI,sulII,catI,catII,ermA,ermB)的种类和累积丰度。在施用生物炭处理中,ARGs含量最低,分别在马铃薯果皮(sull+catI+ermA)和块根(sulI)中积累8.9×102和7.2×102拷贝mL-1。首次研究了利用农业废弃物中的生物炭和蛋壳作为绿色吸附材料降低块茎类蔬菜土壤中抗生素、ARB和ARGs积累风险的可行性。

       Soil contamination with antibiotics and antibiotic resistant bacteria/genes (ARB/ARGs) has becoming an emerging environmental problem. Moreover, the mixed pollutants' transfer and accumulation from soil to tuberous vegetables has posed a great threat against food security and human health. In this work, the application of two absorbing materials (maize biochar and sulfate modified eggshell) was able to reduce the poisonous effect of soil antibiotics on potato root system by stimulate the dissipation of water-soluble antibiotics in soil; and also improve food quality by increasing potato starch, protein, fat, and vitamins. Meanwhile, both amendments could effectively decrease the classes and the accumulative abundance of ARB and ARGs (sulI, sulII, catI, catII, ermA, ermB) in the edible parts of potato. The lowest abundance of ARGs was detected in the biochar application treatment, with the accumulative ARG level of 8.9 × 102 and 7.2 × 102 copies mL-1 in potato peel (sull + catI + ermA) and tuberous root (sulI), respectively. It is the first study to demonstrate the feasibility of biochar and eggshell derived from agricultural wastes as green absorbing materials to reduce soil antibiotic, ARB, and ARGs accumulation risk in tuberous vegetable.

       https://www.sciencedirect.com/science/article/pii/S0269749118301787?via%3Dihub