摘要

    在这项研究中，对从中国七个不同地区的45家猪场废水处理厂（WWTP）收集的水样中的细菌/噬菌体介导的抗生素抗性基因（b / pARGs）进行了监测。我们发现在所有水样中检出了8种主要类型的b / pARGs，其亚型为112种，检出数量在53至92之间。进水口中bARGs和pARGs的绝对丰度高达109拷贝/ mL，且分别为106拷贝/ mL。厌氧缺氧/有氧（AAO）和厌氧短程硝化/反硝化（ASND）处理厂可以有效降低b / pARG的绝对丰度和数量。厌氧处理厂无法降低pARG的绝对丰度，甚至不能增加pARG的数量。流动遗传元件（MGE），细菌群落和环境因素是影响b / pARG谱的重要因素。在这些因素中，细菌群落是影响bARG谱图的主要驱动力，而细菌群落和MGE是影响pARG谱图的主要协同驱动力。这项研究是首次大规模研究中国养猪场污水处理厂中b / pARGs的概况，为农业环境中ARG污染的预防和控制提供参考。

    In this study, the profiles of bacteria/phage-comediated antibiotic resistance genes (b/pARGs) were monitored in water samples collected from 45 pig farm wastewater treatment plants (WWTPs) in seven different regions of China. We found that 8 major types and 112 subtypes of b/pARGs were detected in all the water samples, and the detected number ranged from 53 to 92. The absolute abundances of bARGs and pARGs in the influent were as high as 109 copies/mL and 106 copies/mL, respectively. Anaerobic anoxic/oxic (AAO) and anaerobic short-cut nitrification/denitrification (ASND) treatment plants can effectively reduce the absolute abundance and amount of b/pARGs. Anaerobic treatment plants cannot reduce the absolute abundance of pARGs, and even increase the amount of pARGs. Mobile genetic elements (MGEs), bacterial communities and environmental factors were important factors impacting the b/pARG profile. Among these factors, the bacterial community was the major driver that impacted the bARG profile, while bacterial community and MGEs were the major codrivers impacting the pARG profile. This study was the first to investigate the profiles of b/pARGs in pig farm WWTPs in China on such a large scale, providing a reference for the prevention and control of ARG pollution in agricultural environments.

    https://www.sciencedirect.com/science/article/abs/pii/S0304389420321397