摘要

    为了探索温度对污泥厌氧消化（AD）过程中抗生素抗性基因（ARG）的影响，污泥ARG，质粒ARG和无细胞ARG在嗜温（25°C和35°C）和嗜热（对55°C的消化池进行了研究。研究表明，嗜热消化池中污泥ARGs和质粒ARGs的丰度明显低于嗜温消化池，而嗜热消化池中无细胞ARGs的丰度与嗜温消化池相似。与嗜温消化池相比，嗜热消化池具有较高的古细菌丰度，较低的细菌丰度和不同的微生物群落。污泥可能是污泥AD中潜在的ARG的主要宿主。独特的微生物群落是嗜热污泥AD中ARGs含量低的主要原因。在55°C的高温操作而非中温操作更有利于控制污泥厌氧消化中的ARGs。

    For exploring the impact of temperature on antibiotic resistant genes (ARGs) during sludge anaerobic digestion (AD), the dynamic variations of sludge ARGs, plasmid ARGs, and cell-free ARGs in mesophilic (25 °C and 35 °C) and thermophilic (55 °C) digesters were investigated. The study revealed that the abundance of sludge ARGs and plasmid ARGs in thermophilic sludge AD was significantly lower than that in mesophilic digesters, while the cell-free ARGs abundance of the thermophilic digesters was similar to mesophilic digesters. Higher archaea abundance, lower bacteria abundance, and different microbial community were found in thermophilic digesters compared to that of mesophilic ones. Firmicutes might be a main group of potential hosts of ARGs in sludge AD. The distinct microbial community was the main contributor to the low ARGs abundance in thermophilic sludge AD. Thermophilic operation at 55 °C rather than mesophilic operation is more conducive to control ARGs in sludge anaerobic digestion.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852421002637