摘要

    抗生素抗性基因（ARG）被认为是新兴的污染物，对生态环境和全球人类健康构成越来越大的威胁。在尾矿池中对ARG进行分析对于更好地了解其时空动态至关重要。在这项研究中，高通量定量聚合酶链反应（PCR）技术用于研究尾矿池中ARG的发生，多样性，丰度和分布。在WK0，WK1，WK2和HS中分别检测到97、52、44和56个ARG，还分别检测到11、6、3和6个移动遗传元件（MGE）。池塘水中ARG的绝对丰度范围为6.39×107至1.75×108个拷贝·L-1。另外，WK1和WK2中MGE的含量高于ARG，表明水平基因转移（HGT）的潜力。此外，铜，TOC和MGE与ARGs显着相关。确实，冗余分析（RDA）表明，铜，TOC和MGE解释了ARG分布的61.64％的变化，这暗示了它们在尾矿池ARG的传播和进化中的潜在作用。

    Antibiotic resistance genes (ARGs) are considered emerging contaminants posing an increasing threat to the ecological environment and global human health. Profiling ARGs in tailings ponds is essential to better understand their spatial and temporal dynamics. In this study, high-throughput quantitative polymerase chain reaction (PCR) techniques were used to investigate the occurrence, diversity, abundance, and distribution of ARGs in a tailings pond. A total of 97, 52, 44, and 56 ARGs were detected in WK0, WK1, WK2, and HS, respectively, with 11, 6, 3, and 6 mobile genetic elements (MGEs) also being detected, respectively. The absolute abundance of ARGs in the pond water ranged from 6.39×107 to 1.75×108 copies·L-1. Additionally, the abundance of MGEs were higher than ARGs in WK1 and WK2, indicating the potential for horizontal gene transfer (HGT). Furthermore, Cu, TOC, and MGEs were significantly associated with ARGs. Indeed, redundancy analysis (RDA) revealed that Cu, TOC, and MGEs explained 61.64% of the alteration of the ARG profiles, implying their potential roles in the spread and evolution of ARGs in tailings ponds.

    https://europepmc.org/article/med/33742870