摘要

    城市景观水域中的致病细菌和抗生素抗性基因（ARG）可能对人类健康构成潜在威胁。然而，对于由再生水（RW）和地表水（SW）补充的城市景观水中它们的发生的研究仍然不足。从16个rRNA基因的高通量测序中，从RW或SW补充的六个城市景观水中收集的水样用于分析细菌多样性，并通过定量PCR阵列检测18个ARG和2个整合素整合酶基因。结果表明，Proteobacteria是所有六个城市景观水域中的主要门。 RW补充的城市景观水域细菌物种丰富度低于SW。磺酰胺抗性基因（sulI和sulIII）是这些城市景观水域中的主要ARG。在补充RW和补充SW的城市景观水之间，未观察到磺酰胺抗性基因，四环素抗性基因和大多数β-内酰胺抗性基因的相对丰度的显着差异。相比之下，RW补充的城市景观水域中blaampC基因和qnrA基因的相对丰度显着高于SW补充的城市景观水域中的相对丰度（p <0.05），这表明RW的使用可能会增加特定ARGs的数量。到城市景观水域。有趣的是，在六个城市景观水域中，RW补充的城市景观水域的ARG相对丰富（18个ARG中的12-15个），但相对丰富的ARG相对较低（458.90-1944.67份/ 16S×106）。发现RW补充对城市景观水的细菌多样性和ARG的流行有一定的影响，这为RW补充对城市景观水的作用提供了新的见解。

    Pathogenic bacteria and antibiotic resistance genes (ARGs) in urban landscape waters may pose a potential threat to human health. However, the investigation of their occurrence in the urban landscape waters replenished by reclaimed water (RW) and surface water (SW) is still insufficient. The water samples collected from six urban landscape waters replenished by RW or SW were used to analyze bacterial diversity using high-throughput sequencing of 16S rRNA gene and to detect 18 ARGs and 2 integron-integrase genes by means of quantitative PCR array. Results indicated that Proteobacteria was the dominant phylum in all six urban landscape waters. The bacterial species richness was lower in urban landscape waters replenished by RW than that by SW. Sulfonamide resistance genes (sulI and sulIII) were the major ARGs in these urban landscape waters. No significant difference in the relative abundance of sulfonamide resistance genes, tetracycline resistance genes, and most of beta-lactam resistance genes was observed between RW-replenished and SW-replenished urban landscape waters. By contrast, the relative abundance of blaampC gene and qnrA gene in RW-replenished urban landscape waters was significantly higher than that in SW-replenished urban landscape waters (p < 0.05), which suggested that use of RW may increase the amount of specific ARGs to urban landscape waters. Interestingly, among six urban landscape waters, RW-replenished urban landscape waters had a relatively rich variety of ARGs (12-15 of 18 ARGs) but a low relative abundance of ARGs (458.90-1944.67 copies/16S × 106). The RW replenishment was found to have a certain impact on the bacterial diversity and prevalence of ARGs in urban landscape waters, which provide new insight into the effect of RW replenishment on urban landscape waters.

    https://link.springer.com/article/10.1007/s11356-021-13376-w