摘要

    尽管已经开发了光驱动高级氧化工艺（AOP）来处理废水，但是很少有研究调查AOP同时去除抗生素抗性细菌（ARB），抗生素抗性基因（ARGs）和微污染物（MPs）的可行性。这项研究采用了改进的光芬顿法，该方法使用乙二胺-N，N'-二琥珀酸（EDDS）螯合铁（III），从而将反应pH保持在中性范围内。通过细菌细胞培养，qPCR和原子力显微镜评估了同时去除ARB和相关的细胞外（e-ARGs）和细胞内ARGs（i-ARGs）的能力。还通过液相色谱结合质谱法评估了五种MP的去除。发现包含0.1 mM Fe（III），0.2 mM EDDS和0.3 mM过氧化氢（H2O2）的低剂量可有效在30分钟内将ARB降低6对数，在10分钟内将e-ARGs降低6对数。 。 48小时后未发生ARB再生，这表明所提出的工艺是一种有效的ARB消毒剂。此外，在超纯水中处理30分钟后，五个顽固的MP（卡马西平，双氯芬酸，磺胺甲恶唑，甲丙酸和苯并三唑各自的初始浓度均为10μg/ L）被去除> 99％。还使用合成废水和实际的二次废水出水作为基质对改进的光芬顿工艺进行了验证，结果表明应加倍剂量以确保等效去除性能。总体而言，这项研究表明，修改后的过程是一种乐观的“一站式”解决方案，可以同时减轻化学和生物危害。

    Although photo-driven advanced oxidation processes (AOPs) have been developed to treat wastewater, few studies have investigated the feasibility of AOPs to simultaneously remove antibiotic resistant bacteria (ARB), antibiotic resistance genes (ARGs) and micropollutants (MPs). This study employed a modified photo-Fenton process using ethylenediamine-N,N′-disuccinic acid (EDDS) to chelate iron(III), thus maintaining the reaction pH in a neutral range. Simultaneous removal of ARB and associated extracellular (e-ARGs) and intracellular ARGs (i-ARGs), was assessed by bacterial cell culture, qPCR and atomic force microscopy. The removal of five MPs was also evaluated by liquid chromatography coupled with mass spectrometry. A low dose comprising 0.1 mM Fe(III), 0.2 mM EDDS, and 0.3 mM hydrogen peroxide (H2O2) was found to be effective for decreasing ARB by 6-log within 30 min, and e-ARGs by 6-log within 10 min. No ARB regrowth occurred after 48-h, suggesting that the proposed process is an effective disinfectant against ARB. Moreover, five recalcitrant MPs (carbamazepine, diclofenac, sulfamethoxazole, mecoprop and benzotriazole at an initial concentration of 10 μg/L each) were >99% removed after 30 min treatment in ultrapure water. The modified photo-Fenton process was also validated using synthetic wastewater and real secondary wastewater effluent as matrices, and results suggest the dosage should be doubled to ensure equivalent removal performance. Collectively, this study demonstrated that the modified process is an optimistic ‘one-stop’ solution to simultaneously mitigate both chemical and biological hazards.

    https://www.sciencedirect.com/science/article/abs/pii/S0043135421002736