摘要

    在目前的工作中，使用电离辐射，臭氧化和热处理技术进行了头孢菌素C发酵（CEPF）残留物中抗生素的降解和抗生素抗性基因（ARG）的失活。结果表明，三种处理方法均能有效降解头孢菌素C，在100 kGy剂量下辐射去除率达85.5％，在5.2g O3 / L剂量下臭氧氧化率达79.9％，热去除率分别为71.9％和87.3％在60°C和90°C的温度下处理4小时。在未加工的CEPF残留物中检测到了头孢菌素抗性基因tolC，其辐射度降低了74.2％，臭氧化处理降低了64.6％，热处理降低了26.9％–37.1％。 CEPF残基中蛋白质，葡萄糖和乙酸盐的存在对电离辐射对头孢菌素C的降解具有抑制作用，而当抗生素浓度较低时，效果更显着。辐射和热处理后，COD，多糖和蛋白质的总含量略有变化，但通过臭氧处理却大大降低了。初步的技术经济分析表明，以50 kGy（$ 5.2 / m3）的电子束进行电离辐射的运行成本可与热处理（4.3–7.9 / m3）相提并论，后者比臭氧化处理（$ 14.6 / m3）更经济。

    In present work, the degradation of antibiotic and inactivation of antibiotic resistance genes (ARGs) in cephalosporin C fermentation (CEPF) residues were performed using ionizing radiation, ozonation and thermal treatment. The results showed that the three treatment methods could degrade cephalosporin C effectively, with the removal efficiency of 85.5% for radiation at dose of 100 kGy, 79.9% for ozonation at dosage of 5.2 g O3/L, and 71.9% and 87.3% for thermal treatment at 60 °C and 90 °C for 4 h. The cephalosporin resistance gene tolC was detected in the raw CEPF residues, and its abundance was decrease 74.2% by radiation, 64.6% by ozonation and 26.9%–37.1% by thermal treatment respectively. The presence of protein, glucose and acetate in the CEPF residues had inhibitive influence on the degradation of cephalosporin C by ionizing radiation, and the effect was more significant when the antibiotic concentration was lower. The total content of COD, polysaccharides and protein changed slightly after radiation and thermal treatment, while they were decreased greatly by ozonation. The primary techno-economic analysis showed that the operational cost of ionizing radiation by electron beam at 50 kGy ($5.2/m3) was comparable to thermal treatment ($4.3–7.9/m3), which was more economical than ozonation ($14.6/m3).

    https://www.sciencedirect.com/science/article/abs/pii/S030438941931012X