摘要

   这项工作旨在通过高通量定量聚合酶链反应研究在人工湿地中添加不同外源性诱导物（土霉素及其耐药菌）的抗生素抗性基因（ARG）的行为。结果表明，人工湿地具有降低废水中ARGs相对丰度的潜力，总ARGs去除效率可超过60％。出水中的ARGs谱图与进水中的ARGs谱图不同，这并不能直接反映湿地生物膜中主要ARGs的出口。同时，最高水平的ARGs检出量和相对丰度分别为控制系统43和3.35×10-1，处理系统44和6.40×10-1，这意味着在湿地中ARGs的产生是不可避免的，而抗生素废水中的抗生素抗性细菌确实可以促进系统中ARG的丰度。与诱导物的单一作用相比，它们的协同作用对ARGs的相对丰度有更大的影响。

    This work aimed to study the behavior of antibiotic resistance genes (ARGs) in constructed wetlands with different exogenous inducers additions (oxytetracycline and its resistant bacteria) by high-throughput quantitative polymerase chain reaction. Results indicated that constructed wetlands have the potential to reduce ARGs relative abundances in wastewater, and the total ARGs removal efficiency could exceed 60%. ARGs profile in the effluent differed from that in the influent, and that did not directly reflect the export of dominant ARGs in wetland biofilms. Meanwhile, the highest levels of detected numbers and relative abundances of ARGs were 43 and 3.35 × 10−1 for control system and 44 and 6.40 × 10−1 for treatment system, respectively, which meant that ARGs generation in wetlands were inevitable, and antibiotic and antibiotic-resistant bacteria from wastewater could indeed promote ARGs abundance in the system. Compared to the single roles of inducers, their synergistic role had a more significant influence on ARGs relative abundance.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420310518