摘要

    在这项工作中，研究了在猪粪的中温（mAD）和嗜热厌氧消化（tAD）中，存在铜和锌的情况下，抗生素抗性基因（ARGs），重金属抗性基因（MRGs）和intI1的命运。 结果表明，金属减少了滞后阶段的时间。 在mAD期间，Cu对古细菌群落的抑制作用强于Zn，金属抑制了乙酰碎裂性产甲烷菌的生长。 尽管AD后金属的总浓度增加，但它们已转变为稳定状态。 qnrS，sul1，sul2和drfA7的丰度在mAD后提高了1.2–5.7倍，而在tAD之后降低了，这表明tAD可有效去除ARGs。 结构方程模型分析表明intI1对mAD中ARGs变异具有最标准的直接影响（R = 0.85，p <0.01），而MRGs与ARGs的共现对tAD中ARGs变异具有显着的正影响（R = 0.82） ，p <0.01）

    In this work, fate of antibiotic resistance genes (ARGs), heavy metal resistance genes (MRGs) and intI1 were investigated during mesophilic (mAD) and thermophilic anaerobic digestion (tAD) of swine manure with presence of Cu and Zn. Results showed that metal reduced the lag phase time. Cu showed stronger inhibition than Zn on archaea community and metals inhibited the growth of acetoclastic methanogens during mAD. Although total concentration of metals increased after AD, they were transformed into stable state. The abundance of qnrS, sul1, sul2 and drfA7 increased 1.2–5.7 times after mAD, while reduced after tAD, showed that tAD was effective in ARGs removal. Structural equation model analysis suggested that intI1 had the most standardized direct effects on ARGs variation in mAD (R = 0.85, p < 0.01), while the co-occurrence of MRGs with ARGs showed significantly positive influences on ARGs variation in tAD (R = 0.82, p < 0.01).

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420308269