摘要

   抗生素抗性基因（ARGs）在世界范围内被广泛检测到，通常被视为具有环境持久性的新兴污染物。抗生素很容易促进 ARGs 的增殖。然而，环境中ARGs的动态仍然无法使用单一参数进行量化，这对于评估ARGs通过抗生素传播的能力和有效控制ARGs至关重要。基于磺胺类 (SAs) 污染土壤中 ARG 时间曲线确定的定量特征，开发了一个新参数，称为样品与对照的相对面积比 (ΔAR)，并通过定量构效关系 (QSAR) 模型进行验证.结果表明，ΔAR不仅可用于准确量化SAs抗性基因（Suls）随时间的特征，还可用于揭示Suls增殖与重要因素（即浓度和化学结构）之间的关系。此外，基于ΔAR的QSARs模型表明，生物利用度和共轭转移频率，而不是细菌诱导突变的能力，往往是Sul增殖特征的关键过程。因此，ΔAR 是对环境中 ARG 增殖进行环境风险评估的有用参数。

   Antibiotic resistance genes (ARGs) have been widely detected around the world and are generally viewed as emerging pollutants with environmental persistence. The proliferation of ARGs can be easily promoted by antibiotics. However, the dynamics of ARGs in the environment are still unable to be quantified using a single parameter, which is vital to evaluating the ability of ARGs to spread by antibiotics and effectively controlling ARGs. A new parameter, termed the relative area ratio of sample to control (ΔAR), was developed based on the quantitative features determined by ARG-time curves in soils contaminated with sulfonamides (SAs) and verified by quantitative structure-activity relationships (QSARs) models. The results showed that ΔAR can not only be used to accurately quantify the characteristics of SAs resistance genes (Suls) over time but also be applied to reveal the relationships between the proliferation of Suls and important factors (i.e., concentrations and chemical structures). Moreover, the ΔAR-based QSARs model indicated that bioavailability and the frequency of conjugative transfer, rather than the ability of induced mutations in bacteria, tend to be key processes of the characteristics of the proliferation of Suls. Therefore, ΔAR is a useful parameter to perform environmental risk assessments of ARG proliferation in the environment.

  https://www.sciencedirect.com/science/article/abs/pii/S0048969720320295