摘要

  本实验研究了添加微生物接种物 (M) 和氯化铁 (F) 对鸡粪堆肥过程中抗生素抗性基因 (ARG) 命运的影响。 添加 M 和 F 提高了堆肥中的微生物活性并促进了 ARG 的去除，而联合处理取得了最佳效果，尤其是在减少 sul 抗性基因的富集方面。 Tn916/1545 和 intI1 是影响 ARGs 转移的重要遗传元件，Tn916/1545 与厚壁菌门中 tetM、tetW 和 ermQ 的转移密切相关。 京都基因和基因组百科全书功能预测表明 M 和 F 可以减少堆肥产品中膜转运和信号转导分子的丰度。 因此，这些发现表明 M 和 F 的联合应用是一种很有前景的策略，可以潜在地抑制堆肥过程中 ARG 的转移

   This experiment investigated the effect of adding a microbial inoculum (M) and ferric chloride (F) on the fate of antibiotic resistance genes (ARGs) during chicken manure composting. Adding M and F improved the microbial activity in the compost and facilitated the removal of ARGs, whereas the combined treatment achieved the best results, especially in reducing the enrichment of sul resistance genes. Tn916/1545 and intI1 were important genetic elements that affected the transfer of ARGs, and Tn916/1545 was closely related to the transfer of tetM, tetW, and ermQ in Firmicutes. Kyoto Encyclopedia of Genes and Genomes functional predictions indicated that M and F could reduce the abundance of membrane transport and signal transduction molecules in the compost products. Thus, these findings suggest that the combined application of M and F is a promising strategy that could potentially inhibit the transfer of ARGs during composting. 

  https://www.sciencedirect.com/science/article/abs/pii/S0960852420300717