摘要

       微塑料（MPs）和药物是污水中常见的新兴污染物，它们的共存可能对环境产生更大的负面影响。本研究选择四环素 (TC)、氨苄青霉素 (AMP) 和三氯生 (TCS) 来研究抗生素抗性基因 (ARG) 和微生物群落对不同 MP（聚氯乙烯 (PVC)、聚乙烯 (PE)）生物膜（塑料球）的反应.三种药物对PVC和PE的吸附量依次为AMP>TC>TCS。 PE比PVC更有利于微生物附着。 MPs 导致污水中 ARGs 和移动遗传元件 (MGEs) 的总拷贝数增加。重要的是，多药 ARG 和 MGE 在塑料球上富集。此外，TC 和 MP 的同时出现导致传播 ARG 和 MGE 的风险更高。此外，潜在病原菌军团菌、分枝杆菌、奈瑟菌和弓形杆菌在塑料圈中的含量高于污水中的数量，这些细菌可能是 ARGs 和 MGEs 的宿主。这项研究表明，塑料圈可能是污水中 ARGs 和 MGEs 的储存库，并积累了潜在的致病细菌。

       Microplastics (MPs) and pharmaceuticals are common emerging pollutants in sewage, and their coexistence may have more negative effects on the environments. This study chose tetracycline (TC), ampicillin (AMP) and triclosan (TCS) to investigate the responses of antibiotic resistance genes (ARGs) and microbial communities on different MPs (polyvinyl chloride (PVC), polyethylene (PE)) biofilms (plastisphere). The adsorption capacity of three pharmaceuticals on PVC and PE decreased in the order of AMP > TC > TCS. PE was more conducive to microbial attachment than PVC. MPs led to the increase of the total copies of ARGs and mobile genetic elements (MGEs) in the sewage. Importantly, multidrug ARGs and MGEs were enriched on plastisphere. Furthermore, the co-occurrence of TC and MPs led to higher risks of spreading ARGs and MGEs. In addition, potential pathogenic bacteria Legionella, Mycobacterium, Neisseria and Arcobacter were more abundant on plastisphere than those in sewage, and these bacteria might be the hosts for ARGs and MGEs. This study showed that plastisphere could be repositories of ARGs and MGEs in sewage and accumulated potential pathogenic bacteria.

https://www.sciencedirect.com/science/article/abs/pii/S0048969720381948