摘要

       病原菌的存在和抗生素抗性基因 (ARGs) 的传播可能对接收市政污水处理厂 (WWTPs) 出水的河流构成巨大风险。在这项研究中，我们调查了细菌群落和 ARGs 的变化。一个污水处理厂的处理过程，并检查了污水排放对接收河流中细菌群落和 ARGs 的影响。应用焦磷酸测序揭示细菌群落组成，包括潜在的细菌病原体，并使用 Illumina 高通量测序分析 ARGs。结果表明，污水处理厂对潜在病原菌（尤其是布氏弧杆菌）和 ARGs 有较好的去除效率，而且河流下游和上游的细菌群落没有显着差异。在出水口观察到 ARGs，表明污水处理厂出水可能有助于潜在病原菌和 ARGs 在接收河流中的传播。

       The presence of pathogenic bacteria and the dissemination of antibiotic resistance genes (ARGs) may pose big risks to the rivers that receive the effluent from municipal wastewater treatment plants (WWTPs). In this study, we investigated the changes of bacterial community and ARGs along treatment processes of one WWTP, and examined the effects of the effluent discharge on the bacterial community and ARGs in the receiving river. Pyrosequencing was applied to reveal bacterial community composition including potential bacterial pathogen, and Illumina high-throughput sequencing was used for profiling ARGs. The results showed that the WWTP had good removal efficiency on potential pathogenic bacteria (especially Arcobacter butzleri) and ARGs. Moreover, the bacterial communities of downstream and upstream of the river showed no significant difference. However, the increase in the abundance of potential pathogens and ARGs at effluent outfall was observed, indicating that WWTP effluent might contribute to the dissemination of potential pathogenic bacteria and ARGs in the receiving river.
https://www.sciencedirect.com/science/article/abs/pii/S0147651316302226