摘要

猪粪被认为是抗生素抗性基因( ARGs )的储存库，但在猪粪与中草药残渣( CMHRs )共堆肥过程中ARGs的变化却鲜为人知。因此，本研究探讨了CMHRs与猪粪共堆肥过程中ARG含量变化的影响。结果表明，CMHRs能有效降低大部分靶ARGs ( 0.18～2.82 logs)和移动遗传元件( MGEs ) ( 0.47～3.34 logs)。相关分析表明，CMHRs可能通过水平基因转移降低ARGs的扩散。冗余分析表明，细菌群落对ARGs变异的影响大于环境因子和MGEs。研究结果表明，施用CMHRs可降低农田施用堆肥过程中精氨酸和MGEs的含量，降低ARGs扩散的风险。

Swine manure is considered to be a reservoir for antibiotic resistance genes (ARGs) but little is known about the variations in ARGs during the co-composting of swine manure with Chinese medicinal herbal residues (CMHRs). Thus, this study explored the effects of CMHRs on the variations in ARGs during co-composting with swine manure. The results showed that CMHRs could reduce effectively most of the targeted ARGs (0.18–2.82 logs) and mobile genetic elements (MGEs) (0.47–3.34 logs). The correlations indicated that CMHRs might decrease the spread of ARGs via horizontal gene transfer. Redundancy analysis showed that the bacterial communities had more important effects on the variations in ARGs compared with environmental factors and MGEs. The results of this study demonstrate that CMHRs can decrease the abundances of ARGs and MGEs, as well as reducing the risk of ARGs spreading during the application of compost products to farmland.

https://www.sciencedirect.com/science/article/pii/S096085241731129X