摘要

       环境细菌和共生细菌保持着种类繁多且在很大程度上未知的抗生素抗性基因 (ARG)，随着时间的推移，可能会被动员并转移到病原体上。 宏基因组学能够对细菌群落进行独立于培养的表征，但结果数据嘈杂且高度碎片化，严重阻碍了以前未描述的 ARG 的识别。 因此我们开发了fARGene，一种直接从鸟枪法宏基因组数据中识别和重建 ARG 的方法。
        Environmental and commensal bacteria maintain a diverse and largely unknown collection of antibiotic resistance genes (ARGs) that, over time, may be mobilized and transferred to pathogens. Metagenomics enables cultivation-independent characterization of bacterial communities but the resulting data is noisy and highly fragmented, severely hampering the identification of previously undescribed ARGs. We have therefore developed fARGene, a method for identification and reconstruction of ARGs directly from shotgun metagenomic data.

https://link.springer.com/content/pdf/10.1186/s40168-019-0670-1.pdf