摘要

       微塑料和抗生素抗性基因（ARGs）是沿海地区常见的两种新兴污染物。然而，关于微塑料和 ARGs 在沿海循环水产养殖系统 (RAS) 中同时出现的信息很少。本研究进行了现场采样和实验室分析，以调查典型 RAS 农场中微塑料和 ARG 的分布。结果表明，所有水样中均检出微塑料，丰度为58～72个/m3。水样中总共 10 个 ARG 的绝对丰度范围为 3.24×105 至 7.83×105 拷贝/毫升，而微塑料样品中的绝对丰度范围为 1.59×109-1.83×109 拷贝/克。微塑料和水的微生物群落在门和属水平上均表现出显着差异。微塑料的微生物群落多样性高于水。包括 tetG、qnrS、sul1、sul2 和 ermF 在内的 ARG 与水和 RAS 农场的微塑料中的细菌群落具有相对更活跃的关系。结果表明，微塑料可能是 RAS 农场中 ARG 的重要储存库。这项研究的结果将为沿海水产养殖系统中微塑料和 ARG 的污染控制和环境管理提供有用的信息。

       Microplastics and antibiotic resistance genes (ARGs) are two kinds of emerging contaminants with frequent detection in coastal regions. However, rare information on co-occurrence of microplastics and ARGs in coastal recirculating aquaculture system (RAS) is available. This study performed field sampling and laboratory analysis to investigate the distribution of microplastics and ARGs in a typical RAS farm. The results showed that microplastics were detected in all water samples with the abundances ranging from 58 to 72 items/m3. Absolute abundances of total 10 ARGs in water samples ranged from 3.24 × 105 to 7.83 × 105 copies/mL while those on microplastic samples were in the range of 1.59 × 109-1.83 × 109 copies/g. Microbial communities of microplastics and water showed significant difference at both phylum and genus levels. Microbial community diversity of microplastics was higher than that of water. ARGs including tetG, qnrS, sul1, sul2, and ermF possessed relatively more active relationships with bacterial community in water and on microplastics of the RAS farm. The results suggested that microplastics might be an important reservoir of ARGs in RAS farms. The findings of this study will provide useful information on pollution control and environmental management for both microplastics and ARGs in coastal aquaculture systems.

https://www.sciencedirect.com/science/article/abs/pii/S0147651319309625