摘要

       通过实时定量 PCR (qPCR) 在从 80 名健康人的粪便中分离的噬菌体 DNA 中分析了一组抗生素抗性基因 (ARG)（blaTEM、blaCTX-M-1、mecA、armA、qnrA 和 qnrS）。 77% 的样本在噬菌体 DNA 中对一个或多个 ARG 呈阳性。 blaTEM、qnrA 和 blaCTX-M-1 含量最高，armA、qnrS 和 mecA 较少见。 携带 ARGs 的游离噬菌体可能有助于 ARGs 在肠内和肠外环境中的动员。 

       A group of antibiotic resistance genes (ARGs) (blaTEM, blaCTX-M-1, mecA, armA, qnrA, and qnrS) were analyzed by real-time quantitative PCR (qPCR) in bacteriophage DNA isolated from feces from 80 healthy humans. Seventy-seven percent of the samples were positive in phage DNA for one or more ARGs. blaTEM, qnrA, and, blaCTX-M-1 were the most abundant, and armA, qnrS, and mecA were less prevalent. Free bacteriophages carrying ARGs may contribute to the mobilization of ARGs in intra- and extraintestinal environments.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910789/