摘要

       对暴露于抗生素和金属的微生物中抗生素耐药性的共同选择提出了担忧。作为处理抗生素和含重金属废水的一种有前景的方法，生物电化学系统（BES）可用于去除抗生素和重金属。该研究确定了 BES 中抗生素抗性基因 (ARG) 在暴露于氯霉素 (CAP) 和 Cu2+ 时的命运。分析了编码外排泵（cmlA、floR 和 tetC）、1 类整合子整合酶编码基因和 sul1 基因的 ARG。结果表明CAP和不同浓度Cu2+的共存对ARGs的相对丰度有显着影响。细菌群落结构的变化以及细菌群落与ARGs之间的定量相关分析结果证实，潜在宿主的变化是ARGs变化的关键原因。这项研究揭示了在抗生素和重金属的共同选择压力下 BES 中 ARGs 变异的机制。

       Concerns have been raised regarding co-selection for antibiotic resistance among microorganisms exposed to antibiotics and metals. As a promising approach for treating antibiotics and heavy metal-containing wastewater, a bio-electrochemical system (BES) can be used for antibiotic and heavy metal removal. This study determined the fate of antibiotic resistance genes (ARGs) in a BES when exposed to chloramphenicol (CAP) and Cu2+. The ARGs encoding the efflux pump (cmlA, floR, and tetC), the class 1 integron integrase-encoding gene, and the sul1 gene were analyzed. The results indicated that the co-existence of CAP and different concentrations of Cu2+ had significant influence on the relative abundances of the ARGs. The changes in the bacterial community structure and the results of a quantitative correlation analysis between the bacterial community and the ARGs confirmed that the shift in the potential hosts was the key reason for the changes of the ARGs. This study sheds new light on the mechanisms of ARGs variations in BES under the co-selection pressure of antibiotics and heavy metals.

https://www.sciencedirect.com/science/article/pii/S0160412018328320