摘要

       通过 qPCR 在环境水样的噬菌体和细菌 DNA 部分中量化了六种抗生素抗性基因（blaCTX-M、blaSHV、blaTEM、qnrA、qnrB 和 qnrS），以确定噬菌体对抗生素抗性基因传播的贡献。 ARGs）在环境中。 尽管在细菌 DNA 部分中检测到 ARG 的最高拷贝数 (p <0.05)，但在所有分析样本的噬菌体 DNA 中都发现了 qnrS 和 blaSHV 基因，在医院样本中高达 4 log10 拷贝数/mL。 这些结果表明，噬菌体是一个潜在的抗性基因库，可以作为水平基因转移的有效载体。

       Six antibiotic resistance genes (blaCTX-M, blaSHV, blaTEM, qnrA, qnrB and qnrS) were quantified by qPCR in both phage and bacterial DNA fractions of environmental water samples in order to determine the contribution of phages to the dissemination of antibiotic resistance genes (ARGs) in the environment. Although the highest copy numbers (p <0.05) of ARGs were detected in the bacterial DNA fraction, qnrS and blaSHV genes were found in the phage DNA from all samples analysed, reaching up to 4 log10 copy numbers/mL in hospital samples. These results indicate that bacteriophages are a potential reservoir of resistance genes and may act as efficient vehicles for horizontal gene transfer.

https://www.sciencedirect.com/science/article/pii/S1198743X14611745