摘要

       由于有机食品的市场不断扩大，以粪肥为基础的商业有机肥料 (COF) 的应用正变得越来越广泛。本研究通过土壤微观实验研究了重复施用基于鸡或猪粪的 COF 对土壤中抗生素和抗生素抗性基因 (ARG) 命运的影响。 COFs 的应用显着增加了抗生素残留，以及土壤中 ARGs 和 1 类整合子 (intl1) 整合酶基因的相对丰度。每次施用两个月后，抗生素和 ARG 在改良土壤中消散，但与对照相比，它们仍保持在较高水平。而且，由于重复使用 COF，发现了抗生素的积累。然而，大多数 COF 修正土壤中 ARGs 的相对丰度在第一次施用和重复施用之间没有显着差异。结果意味着 2 个月不足以使 ARG 接近背景水平，并且在将其用于农业生态系统之前必须更有效地处理动物粪便。

       The application of manure-based commercial organic fertilizers (COFs) is becoming increasingly extensive because of the expanding market for organic food. The present study examined the effects of repeated applications of chicken or swine manure-based COFs on the fate of antibiotics and antibiotic resistance genes (ARGs) in soil by conducting a soil microcosm experiment. Application of COFs significantly increased antibiotics residues, as well as the relative abundance of ARGs and the integrase gene of class 1 integrons (intΙ1) in soil. Two months after each application, antibiotics and ARGs dissipated in amended soils, but they still remained at an elevated level, compared with the control. And, the accumulation of antibiotics was found due to repeated COF applications. However, the relative abundance of ARGs in most COF-amended soils did not differ significantly between the first application and the repeated application. The results imply that 2 months are not sufficient for ARGs to approach background levels, and that animal manure must be treated more effectively prior to using it in agriculture ecosystems.

https://link.springer.com/article/10.1007/s11356-016-7854-z