摘要

       抗生素抗性基因 (ARG) 是新出现的环境污染物，对公众健康构成威胁。本研究对4个城市污水和8个具有不同污水处理能力和处理工艺的农村生活污水处理系统中的4个四环素抗性基因（tetM、tetO、tetQ和tetW）和两个磺胺类抗性基因（sulI和sulII）进行定量评价。聚合酶链反应 (qPCR)。在流入物中，不同 ARG 的相对丰度在采样点之间显示出显着差异。此外，还观察到基因拷贝数与废水接收能力之间存在显着相关性（tetQ：R2 = 0.712，P < 0.05；tetO：R2 = 0.394，P < 0.05）。统计分析显示 sulI 和 intI1 的基因拷贝数之间呈正相关（R2 = 0.756，P < 0.05），而 tetM 和 sulI 的基因数与 16S rDNA 强相关。在市政污水处理系统中观察到 ARG 的显着减少（1-3 个数量级），但在农村生活污水处理系统中发现的减少较小。这些结果为了解中国东部农村和城市地区废水处理系统中 ARGs 的发生和去除提供了见解。

       Antibiotic resistance genes (ARGs) are emerging environmental contaminants and pose a threat to public health. In this study, four tetracycline resistance genes (tetM, tetO, tetQ and tetW) and two sulfonamide resistance genes (sulI and sulII) were evaluated in 4 municipal wastewater and 8 rural domestic sewage treatment systems with different wastewater handling abilities and treatment processes using quantitative polymerase chain reaction (qPCR). In the influents, the relative abundance of different ARGs showed significant variations among the sampling sites. In addition, significant correlations (tetQ: R2 = 0.712, P < 0.05; tetO: R2 = 0.394, P < 0.05) between the gene copy numbers and wastewater-receiving capacity were observed. Statistical analysis revealed a positive correlation (R2 = 0.756, P < 0.05) between the gene copy numbers of sulI and intI1, whereas the gene numbers of tetM and sulI were strongly correlated with 16S rDNA. Significant reductions (1–3 orders of magnitude) in ARGs were observed in municipal wastewater treatment systems, but a smaller reduction was found in the rural domestic sewage treatment systems. These results provide insights into the occurrence and removal of ARGs in wastewater treatment systems in both rural and urban areas in eastern China.

https://www.sciencedirect.com/science/article/pii/S0160412013000391