摘要 

       由于抗生素在全球范围内的过度使用，抗生素抗性基因 (ARG) 已被普遍报道。抗生素过度使用会扰乱环境并威胁公众健康。本研究的目的是测量四环素组 (TC) 中兽用抗生素的残留浓度，包括四环素 (TC) 和金霉素 (CTC)，以及磺胺组 (SA) 中的兽用抗生素残留浓度，包括磺胺二甲嘧啶 (SMT) 、磺胺甲恶唑 (SMX) 和磺胺噻唑 (STZ)。我们还在农业生态系统中分离了相应的 ARG。四个沉积物样品和两个稻田土壤样品是从韩国洪川奈仁川河沿岸的猪堆肥设施附近的地点收集的。采用高效液相色谱-串联质谱 (HPLC-MS/MS) 和固相萃取方法来测量每种抗生素的浓度。使用合成引物通过定性聚合酶链反应鉴定 ARG。在沉积物样品中高度检测到 SA 及其相应的 ARG，而除沉积物样品 #1 外未检测到 TC。在稻田土壤中检测到 TCs 和 SAs 的 ARGs，而 TCs 的 ARGs 仅在沉积物 #2 和 #4 中发现。需要持续监测抗生素残留及其对环境的综合影响，以确保环境健康。

       Antibiotic resistance genes (ARGs) have been commonly reported due to the overuse worldwide of antibiotics. Antibiotic overuse disturbs the environment and threatens public human health. The objective of this study was to measure the residual concentrations of veterinary antibiotics in the tetracycline group (TCs), including tetracycline (TC) and chlortetracycline (CTC), as well as those in the sulfonamide group (SAs), including sulfamethazine (SMT), sulfamethoxazole (SMX), and sulfathiazole (STZ). We also isolated the corresponding ARGs in the agroecosystem. Four sediment samples and two rice paddy soil samples were collected from sites near a swine composting facility along the Naerincheon River in Hongcheon, Korea. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed with a solid-phase extraction method to measure the concentration of each antibiotic. ARGs were identified by the qualitative polymerase chain-reaction using synthetic primers. SAs and their corresponding ARGs were highly detected in sediment samples whereas TCs were not detected except for sediments sample #1. ARGs for TCs and SAs were detected in rice paddy soils, while ARGs for TCs were only found in sediment #2 and #4. Continuous monitoring of antibiotic residue and its comprehensive impact on the environment is needed to ensure environmental health.

https://www.hindawi.com/journals/jchem/2015/974843/