摘要

       转基因 (GM) 棉花生产会产生大量的秸秆，而且处理起来很困难。为了研究使用转基因棉秆堆肥的可行性以及堆肥过程中抗生素抗性基因（ARGs）发生的变化，我们在堆肥过程中用转基因或非转基因棉秆补充猪粪，并比较了它们对绝对值的影响。两种处理下 intI1、intI2 和 ARGs 的丰度（AA）。根据发芽指数和C/N比处理后的堆肥成熟。堆肥后，在非转基因和转基因处理中，ARGs、intI1 和 intI2 的 AA 分别降低了 41.7% 和 45.0%。 ARG 曲线受温度和氨氮显着影响。此外，不包括 tetC，与非转基因处理相比，转基因棉秆对 ARGs、intI1 和 intI2 没有显着影响（p < 0.05）。因此，与非转基因棉秆相似，转基因棉秆可与牲畜粪便进行好氧堆肥，并且可以降低 ARGs 的 AAs。此外，本研究结果为转基因棉秆的无害化利用提供了理论依据。

       Genetically modified (GM) cotton production generates a large yield of stalks and their disposal is difficult. In order to study the feasibility of using GM cotton stalks for composting and the changes that occur in antibiotic resistance genes (ARGs) during composting, we supplemented pig manure with GM or non-GM cotton stalks during composting and we compared their effects on the absolute abundances (AA) of intI1, intI2, and ARGs under the two treatments. The compost was mature after processing based on the germination index and C/N ratio. After composting, the AAs of ARGs, intI1, and intI2 were reduced by 41.7% and 45.0% in the non-GM and GM treatments, respectively. The ARG profiles were affected significantly by temperature and ammonia nitrogen. In addition, excluding tetC, GM cotton stalks had no significant effects on ARGs, intI1, and intI2 compared with the non-GM treatment (p < 0.05). Thus, similar to non-GM cotton stalks, GM cotton stalks can be used for aerobic composting with livestock manure, and the AAs of ARGs can be reduced. Furthermore, the results of this study provide a theoretical basis for the harmless utilization of GM cotton stalks.

https://www.sciencedirect.com/science/article/abs/pii/S0147651317305924