摘要 

       本研究检查了 23 种代表性抗生素抗性基因 (ARG) 的命运，这些基因编码四环素类、磺胺类、喹诺酮类、β-内酰胺类抗生素、大环内酯类、氟苯尼考和污水污泥的嗜热好氧消化 (TAD) 期间的多药耐药性。 还量化了细菌群落、1 类整合子 (intI1) 和四种金属抗性基因 (MRG)，以确定 TAD 期间 ARG 变化的关键驱动因素。 在消化结束时，观察到 ARGs、MRGs 和 intI1 以及 16S rRNA 基因的数量显着减少。 部分冗余分析 (RDA) 表明，温度变化是影响 ARG 减少的关键因素。 温度变化通过减少抗性组和细菌多样性导致 ARG 数量减少，而不是通过 intI1 降低水平转移电位或通过 MRG 降低共抗性。
       This study examines the fate of twenty-three representative antibiotic resistance genes (ARGs) encoding tetracyclines, sulfonamides, quinolones, β-lactam antibiotics, macrolides, florfenicol and multidrug resistance during thermophilic aerobic digestion (TAD) of sewage sludge. The bacterial community, class 1 integrons (intI1) and four metal resistance genes (MRGs) were also quantified to determine the key drivers of changes in ARGs during TAD. At the end of digestion, significant decreases in the quantities of ARGs, MRGs and intI1 as well as 16S rRNA genes were observed. Partial redundancy analysis (RDA) showed that shifts in temperature were the key factors affecting a decrease in ARGs. Shifts in temperature led to decreased amounts of ARGs by reducing resistome and bacterial diversity, rather than by lowering horizontal transfer potential via intI1 or co-resistance via MRGs.

https://www.sciencedirect.com/science/article/abs/pii/S0960852417319028