摘要

       厌氧膜生物反应器 (AnMBR) 是一种新兴技术，具有提高主流废水处理中的能源效率和废水再利用的潜力。然而，它们对新出现的污染物扩散的贡献，如抗生素抗性基因 (ARG)，在很大程度上仍然未知。本研究的目的是确定不同浓度的选择流入抗生素对 AnMBR 系统及其流出物中 ARG 的存在和丰度的影响。靶向 ARG 的量化揭示了生物质和流出物的不同特征，基因赋予对不同抗生素类别的抗性在生物质（大环内酯类）和流出物（磺胺类）中占主导地位。流出物 sul1 基因丰度与 intl1 丰度密切相关，表明可移动遗传元件在 AnMBR 系统释放 ARG 中的潜在重要性。添加特定抗生素也影响了其相关 ARG 的标准化丰度，举例说明了选择压力在低 (10 μg/L) 和高 (250 μg/L) 进水抗生素浓度下的潜在影响。

       Anaerobic membrane bioreactors (AnMBRs) are an emerging technology with potential to improve energy efficiency and effluent reuse in mainstream wastewater treatment. However, their contribution to the proliferation of contaminants of emerging concern, such as antibiotic resistance genes (ARGs), remains largely unknown. The purpose of this study was to determine the effect of select influent antibiotics at varying concentrations on the presence and abundance of ARGs in an AnMBR system and its effluent. Quantification of targeted ARGs revealed distinct profiles in biomass and effluent, with genes conferring resistance to different antibiotic classes dominating in biomass (macrolides) and effluent (sulfonamides). Effluent sul1 gene abundance was strongly correlated with abundance of intl1, signifying the potential importance of mobile genetic elements in ARG release from AnMBR systems. The addition of specific antibiotics also affected normalized abundances of their related ARGs, exemplifying the potential impact of selective pressures at both low (10 μg/L) and high (250 μg/L) influent antibiotic concentrations.

https://pubs.acs.org/doi/abs/10.1021/acs.est.9b00798