摘要

       研究了痕量浓度 (1ug L-1) 的两种抗生素 - 诺氟沙星 (NOR) 和红霉素 (ERY) 对厌氧氨氧化生物膜的长期影响。通过批量实验检测厌氧氨氧化生物膜的厌氧氨氧化比活性（SAA）和脱氢酶活性（DHA），采用高通量测序技术分析微生物多样性，通过qPCR检测抗生素抗性基因（ARGs）。结果表明，长期 NOR 饲喂降低了 30% SAA 和 39.6% DHA，并且还降低了与自养脱氮相关的 OTU 的丰度，而 ERY 对厌氧氨氧化的影响很小。在两个 Anammox 系统中仅检测到两个靶向 ERY（ermB、mphA）的 ARG，而未检测到靶向 NOR（qnrA、qnrB、qnrD 和 qnrS）的 ARG。 ermB与16S rRNA的相对表达量从2.08±0.32×10-4增加到3.53±1.18×10-4，mphA从4.48±1.32×10-5增加到5.00±0.48×10-4。 Anammox 系统中诱导的 ARG 有助于它抵抗 ERY 冲击。

       Long-term impacts of two antibiotics-norfloxacin (NOR) and erythromycin (ERY) in trace concentration (1ug L−1) on Anammox biofilm were investigated. The specific Anammox activity (SAA) and dehydrogenase activity (DHA) of Anammox biofilm were detected by batch experiments, the microbial diversity was analyzed using high-throughput sequencing technology and the antibiotic resistance genes (ARGs) were measured by qPCR. Results showed that long-term NOR feeding decreased 30% SAA and 39.6% DHA, and also decreased the abundance of the OTUs related to autotrophic nitrogen removal, while ERY had slight impact on Anammox. Only two ARGs targeted to ERY (ermB, mphA) were detected in the two Anammox systems while those targeted to NOR (qnrA, qnrB, qnrD, and qnrS) were not detected. The relative expression of ermB to 16S rRNA increased from 2.08±0.32×10-4 to 3.53±1.18×10-4, and that of mphA increased to 5.00±0.48×10-4 from 4.48±1.32×10-5. The induced ARGs in the Anammox system help it resist the ERY shock.

https://www.sciencedirect.com/science/article/abs/pii/S0304389418312317