摘要

       抗生素抗性基因 (ARG) 已被视为新兴污染物，并因其在环境中的广泛存在而引起越来越多的关注。除了有据可查的抗生素选择压力外，ARGs 也因人为影响而变得普遍。沿海栖息地位于陆地和海洋生态系统之间，是人为影响的热点。多环芳烃 (PAHs) 的过度积累对沿海栖息地构成了严重威胁，但没有关于多环芳烃对沿海环境微生物群落抗生素耐药性影响的信息。在这项研究中，在沿海微生物群落中研究了两种典型的多环芳烃，萘和菲，对抗生素耐药性传播的影响。结果表明，100 mg/L萘或10 mg/L菲的存在显着提高了微生物中I类整合酶基因（intI1）、磺胺类抗性基因（sulI）和氨基糖苷类抗性基因（aadA2）的丰度。社区。水平基因转移实验表明 ARGs 丰度的增加主要是由 I 类整合子介导的接合转移的结果。这些发现提供了直接证据，表明暴露于多环芳烃的沿海微生物群落可能导致 ARG 的传播，并暗示有必要对多环芳烃对自然生态系统和公共卫生进行更全面的风险评估。

       Antibiotic resistance genes (ARGs) have been regarded as emerging contaminants and have attracted growing attention owing to their widespread presence in the environment. In addition to the well-documented selective pressure of antibiotics, ARGs have also become prevalent because of anthropogenic impacts. Coastal habitats are located between terrestrial and marine ecosystems, which are a hotspot for anthropogenic impacts. Excessive accumulation of polycyclic aromatic hydrocarbons (PAHs) has posed a serious threat to coastal habitats, but no information is available on the effect of PAHs on antibiotic resistance in the microbial community of coastal environments. In this study, the effect of two typical PAHs, naphthalene and phenanthrene, on antibiotic resistance propagation was investigated in a coastal microbial community. The results indicated that the presence of 100 mg/L of naphthalene or 10 mg/L of phenanthrene significantly enhanced the abundance of class I integrase gene (intI1), sulfanilamide resistance gene (sulI), and aminoglycosides resistance gene (aadA2) in the microbial community. Horizontal gene transfer experiment demonstrated that increased abundance of ARGs was primarily a result of conjugative transfer mediated by class I integrons. These findings provided direct evidence that coastal microbial community exposed to PAHs might have resulted in the dissemination of ARGs and implied that a more comprehensive risk assessment of PAHs to natural ecosystems and public health is necessary.

https://www.sciencedirect.com/science/article/abs/pii/S0269749117319863