摘要

       这项研究评估了厌氧细菌多样性与抗生素组合的生物降解之间的联系，并评估了修改抗生素组合和逐步增加抗生素浓度如何影响厌氧反应器中抗性基因的发展。分别使用UV-HPLC和qPCR分析过程研究了红霉素和四环素的已知抗生素抗性基因(ARG)的生物降解、吸附和发生。 Ion Torrent 测序用于检测响应于添加抗生素的微生物群落变化。总体结果表明，微生物群落结构的变化会导致生物降解能力、抗生素组合的吸附和 ARG 的发生发生变化。增强的生物降解效率似乎会导致细菌群落结构发生变化。结果表明，控制最终的革兰氏阴性菌群落，尤其是不动杆菌相关种群，可以促进抗生素组合的成功生物降解并减少 ARGs 的发生。

       This study evaluated the link between anaerobic bacterial diversity and, the biodegradation of antibiotic combinations and assessed how amending antibiotic combination and increasing concentration of antibiotics in a stepwise fashion influences the development of resistance genes in anaerobic reactors. The biodegradation, sorption and occurrence of the known antibiotic resistance genes (ARGs) of erythromycin and tetracycline were investigated using the processes of UV-HPLC and qPCR analysis respectively. Ion Torrent sequencing was used to detect microbial community changes in response to the addition of antibiotics. The overall results indicated that changes in the structure of a microbial community lead to changes in biodegradation capacity, sorption of antibiotics combinations and occurrence of ARGs. The enhanced biodegradation efficiency appeared to generate variations in the structure of the bacterial community. The results suggested that controlling the ultimate Gram-negative bacterial community, especially Acinetobacter-related populations, may promote the successful biodegradation of antibiotic combinations and reduce the occurrence of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0960852416300566