摘要

       研究了来自两种不同类型水产养殖场的沉积物的细胞外和细胞内 DNA（eARG 和 iARG）中 ARG 的发生和数量。 共鉴定出与 7 类常用抗生素（如氨基糖苷类、β-内酰胺类、磺胺类、四环素类）相关的 20 个 ARG 亚型，其中一些亚型与使用的抗生素无关。 ARGs 主要以 iDNA 形式存在，总 iARGs 与 eARGs 的比率在 7.9-45.5 的范围内。 eARG 与 iARG 的比率因 ARG 亚型而异，这可能是由于它们作为 eDNA 的一部分在持久性方面存在差异。 对于来自水产养殖场的沉积物中的 eDNA 和 iDNA，观察到 int1 和 ARG 之间存在显着相关性。 此外，由于在牛蛙养殖过程中更频繁地使用抗生素，牛蛙池塘中的 ARG 污染比混养池塘更严重。

       The occurrence and quantities of ARGs in extracellular and intracellular DNA (eARGs and iARGs) from sediments collected in two different types of aquaculture farms were investigated. A total of 20 ARG subtypes associated with 7 categories of commonly used antibiotics (e.g., aminoglycosides, beta-lactams, sulfonamides, tetracyclines) were identified, and some of these subtypes were not related to the antibiotics used. ARGs are mainly present in the iDNA form with the ratio of the total iARGs to eARGs being in the range of 7.9–45.5. The ratio of eARG to iARGs varies greatly with ARG subtypes, probably due to their differences in persistence as a part of eDNA. Significant correlation between int1 and ARGs was observed for both eDNA and iDNA in sediments from the aquaculture farms. Moreover, ARG pollution was more serious in bullfrog ponds than polyculture ponds due to the more frequent use of antibiotics in bullfrog rearing operations.

https://www.sciencedirect.com/science/article/abs/pii/S0045653519313268