摘要

       研究表明，猪粪是抗生素抗性基因 (ARG) 的储存库。然而，对于饲喂不同日粮的仔猪和成年猪的粪便中 ARGs 的特征知之甚少。在本研究中，使用高通量荧光定量 PCR 分析了饲喂不同日粮（饲料、谷物）的仔猪和成年猪粪便的 ARG 特征。分析了猪粪中重金属、抗生素和 ARGs 之间的相关性。结果表明，饲喂猪粪中重金属和抗生素含量明显高于饲粮猪粪。仔猪粪便中重金属和抗生素含量高于成年猪。饲料显着增加了猪粪中的 ARG 多样性。仔猪粪便中ARG多样性高于成年猪。在饲喂猪的粪便中，与饲喂谷物的猪相比，25（来自仔猪）、12（来自成年猪）ARGs 显着富集。特别是，sat4（在仔猪中）和 vatE-01（在成年猪中）显示出最高的富集度，分别增加了 59 倍和 19 倍。 ARG 多样性与粪便中抗生素和重金属的浓度呈正相关。 

       Studies have shown that pig manure is a repository of antibiotic resistance genes (ARG). However, little is known about the characteristics of ARGs in the feces of piglets and adult pigs fed different diets. In this study, high-throughput fluorescent quantitative PCR was used to analyze the ARG characteristics of piglets and adult pig feces fed different diets (feed, grain). The correlation between heavy metals, antibiotics and ARGs in pig manure was analyzed. The results showed that the content of heavy metals and antibiotics in fed pig manure was significantly higher than that in feed pig manure. The content of heavy metals and antibiotics in piglet manure is higher than that of adult pigs. Feed significantly increases the ARG diversity in pig manure. The ARG diversity in piglet manure is higher than that of adult pigs. Compared with grain-fed pigs, 25 (from piglets) and 12 (from adult pigs) ARGs were significantly enriched in the manure of fed pigs. In particular, sat4 (in piglets) and vatE-01 (in adult pigs) showed the highest degree of enrichment, increasing by 59 times and 19 times, respectively. The diversity of ARG is positively correlated with the concentration of antibiotics and heavy metals in feces
https://www.sciencedirect.com/science/article/abs/pii/S0269749117310321