摘要

       蔬菜中抗生素、重金属、抗生素耐药菌（ARB）/抗生素耐药基因（ARGs）的综合积累已成为对人类健康的新威胁。这是第一项研究硫酸铝改性的煅烧蛋壳作为新型农业废物阻止混合污染物转移到甜椒（Capsicum annuum L.）的可行性的研究。在这项工作中，煅烧蛋壳改良剂显着减轻了甜椒中混合污染物的积累，增强了土壤四环素、磺胺嘧啶、罗红霉素和氯霉素的消散，减少了抗生素的水溶性部分，并降低了蔬菜中 ARB/ARGs 的多样性.此外，定量聚合酶链反应分析检测到甜椒果实中的 ARG 水平显着降低至 10-10 拷贝/16S 拷贝，表明 ARG 沿食物链转移的风险有限。此外，土壤微生物生物学功能的恢复表明，煅烧蛋壳是一种环境友好的改良剂，可控制土壤 ARB/ARGs 在土壤 - 蔬菜系统中的传播。 

       The comprehensive accumulation of antibiotics, heavy metals, and antibiotic-resistant bacteria (ARB)/antibiotic resistance genes (ARGs) in vegetables has become a new threat to human health. This is the first study to investigate the feasibility of using aluminum sulfate modified calcined eggshells as a new type of agricultural waste to prevent the transfer of mixed pollutants to sweet peppers (Capsicum annuum L.). In this work, the calcined eggshell improver significantly reduced the accumulation of mixed pollutants in sweet peppers, enhanced the dissipation of soil tetracycline, sulfadiazine, roxithromycin and chloramphenicol, and reduced the water-soluble part of antibiotics. , And reduced the diversity of ARB/ARGs in vegetables. In addition, quantitative polymerase chain reaction analysis detected that the ARG level in sweet pepper fruits was significantly reduced to 10-10 copies/16S copies, indicating that the risk of ARG transfer along the food chain is limited . In addition, the restoration of the biological functions of soil microorganisms shows that calcined eggshells are an environmentally friendly amendment that can control the spread of soil ARB/ARGs in the soil-vegetable system. 

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.6b00866