摘要

       活性污泥来自分布在三个地理隔离区域的五个污水处理厂 (STP)，即中国的香港（沙田、赤柱）、中国的上海（闵行）和加利福尼亚州的湾区（帕洛阿尔托和圣何塞）。美国。在测试的 14 个四环素抗性 (tet) 基因中，通常检测到 9 个基因，包括外排泵 (tetA、tetC、tetE 和 tetG)、核糖体保护蛋白 (tetM、tetO、tetQ 和 tetS) 和酶促修饰 (tetX)在 STP 污泥样本中，而在任何污泥样本中均未检测到包含外排泵 [tetB、tetD、tetL、tetK 和 tetA(P)] 的五个基因。此外，从沙田污水处理厂的活性污泥中分离出 109 株乳糖发酵肠杆菌科 (LFE) 菌株。四环素抗性 (TR) LFE 占 109 个 LFE 菌株总数的 32%。 tet基因在所有TR-LEF菌株中的出现频率从0%到91%不等，即tetC（91%）、tetA（46%）、tetE（9%）、tetG（6%）和tetD（6%） ）。最后，定量实时聚合酶链反应被用于量化 tetC 和 tetA 基因的变化，作为沙田和赤柱 STP 中 TR-LEF 的指标。结果表明，STP流出液中tetC和tetA基因的浓度范围为104~105copies/mL，明显低于流入液3个数量级。

       Activated sludge comes from five sewage treatment plants (STP) distributed in three geographically isolated areas, namely Hong Kong (Shatin and Stanley) in China, Shanghai (Minhang) in China and the Bay Area in California (Palo Alto and Stanley). San Jose). America. Of the 14 tetracycline resistance (tet) genes tested, 9 genes are usually detected, including efflux pumps (tetA, tetC, tetE, and tetG), ribosomal protective proteins (tetM, tetO, tetQ, and tetS), and enzymes Modification promotion (tetX) was found in STP sludge samples, and no five genes containing efflux pump [tetB, tetD, tetL, tetK and tetA(P)] were detected in any sludge samples. In addition, 109 strains of lactose-fermenting Enterobacteriaceae (LFE) were isolated from the activated sludge of the Shatian Wastewater Treatment Plant. Tetracycline-resistant (TR) LFE accounted for 32% of the total number of 109 LFE strains. The frequency of tet genes in all TR-LEF strains ranges from 0% to 91%, namely tetC (91%), tetA (46%), tetE (9%), tetG (6%) and tetD (6%) ) ). Finally, quantitative real-time polymerase chain reaction was used to quantify the changes in tetC and tetA genes, as an indicator of TR-LEF in STP in Sha Tin and Stanley. The results showed that the concentration of tetC and tetA genes in the STP effluent ranged from 104 to 105 copies/mL, which was significantly lower than the influent by 3 orders of magnitude.

https://pubs.acs.org/doi/abs/10.1021/es803309m