摘要

       环境系统中的移动抗生素抗性基因 (ARG) 可能对公共健康构成威胁。共存的取代芳香族污染物可能有助于 ARG 穿过细胞外聚合物渗透屏障进入细胞内部，促进 ARG 的传播，但其机制尚不清楚。在这里，我们证明了细胞外基质中移动质粒的特定抗水解机制对这种促进传播做出了更大的贡献。具体而言，使用 Tb3+ 标记的 pUC19 质粒进行荧光微量滴定来研究与 ARG 传播相关的取代芳香质粒复合物的形成。 endA 基因和 EPS 的操作证实，这些形成的复合物拮抗 EPS 介导的质粒水解。傅里叶变换红外光谱 (FTIR)、X 射线光电子能谱 (XPS) 和计算化学表明，取代基改变了芳香族分子的极性，使 1, 3-二氯苯的 6 位碳以及不稳定的质子间苯二胺、苯胺和 2-萘酚的 (-NH2/-OH) 与磷酸酯的去质子化羟基 (PO…HC/N/O) 主要通过氢键相互作用。 ARG 传播、结合常数和键合能之间的线性相关性突出了 ARG 增殖对 D-核糖-磷酸模板化功能组合的定量依赖性。

       Mobile antibiotic resistance genes (ARG) in the environmental system may pose a threat to public health. The co-existing substituted aromatic pollutants may help ARG to penetrate the extracellular polymer permeability barrier into the cell and promote the spread of ARG, but the mechanism is still unclear. Here, we prove that the specific anti-hydrolysis mechanism of the mobile plasmid in the extracellular matrix makes a greater contribution to this promotion of propagation. Specifically, the Tb3+-labeled pUC19 plasmid was used for fluorescent microtitration to study the formation of substituted aromatic plasmid complexes related to ARG transmission. The manipulation of endA gene and EPS confirmed that these complexes antagonize EPS-mediated plasmid hydrolysis. Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and computational chemistry show that substituents change the polarity of aromatic molecules, making the 6-position carbon of 1,3-dichlorobenzene and unstable The (-NH2/-OH) of proton meta-phenylenediamine, aniline and 2-naphthol interact with the deprotonated hydroxyl group (PO...HC/N/O) of the phosphate ester mainly through hydrogen bonds. The linear correlation between ARG propagation, binding constant, and bonding energy highlights the quantitative dependence of ARG proliferation on the functional combination of D-ribose-phosphate templated.

https://www.researchgate.net/publication/329768459_Substituted_Aromatic-Facilitated_Dissemination_of_Mobile_ARGs_via_an_Anti-Hydrolysis_Mechanism_across_an_EPS_Permeable_Barrier