摘要

       细菌之间的抗生素耐药性是世界范围内日益严重的问题，污水处理厂被认为是抗生素耐药性向环境传播的主要因素之一。缺乏关于不同季节大量抗生素抗性基因 (ARG) 的综合定量分子数据，其采样策略将涵盖进水和出水以及从过程中去除的多余污泥。为了填补这一空白，我们使用高通量 qPCR 阵列对城市污水处理厂四个季节的 ARG 和水平基因转移潜力进行了高度平行的定量分析。在废水中检测到所有分析的转座酶和三分之二的靶向 ARG 的引物组。大多数基因的相对丰度在进水中最高，在出水和污泥中较低。样品的阻力分布按样品位置聚集，从原始进水到最终出水和干污泥再到沉积物。废水排放仅在排放管附近的沉积物中富集了少数基因，即 Tn25 型转座酶基因和临床 1 类整合子，但这些富集的基因可能表明存在水平基因转移的潜力。

       Antibiotic resistance among bacteria is an increasingly serious problem worldwide, and sewage treatment plants are considered to be one of the main factors in the spread of antibiotic resistance to the environment. There is a lack of comprehensive quantitative molecular data on a large number of antibiotic resistance genes (ARG) in different seasons, and the sampling strategy will cover influent and effluent as well as excess sludge removed from the process. To fill this gap, we used a high-throughput qPCR array to perform a highly parallel quantitative analysis of ARG and horizontal gene transfer potential in the four seasons of urban sewage treatment plants. All the transposases analyzed and two-thirds of the primer sets targeting ARG were detected in the wastewater. The relative abundance of most genes is highest in influent water and lower in effluent and sludge. The resistance distribution of the sample is gathered according to the position of the sample, from the original water inlet to the final water outlet and dry sludge to the sediment. Wastewater discharge only enriched a few genes in the sediments near the discharge pipe, namely the Tn25 type transposase gene and the clinical type 1 integron, but these enriched genes may indicate the potential for horizontal gene transfer. 

https://academic.oup.com/femsec/article/92/3/fiw014/2470099?login=true