摘要
      这项工作研究了抗生素抗性基因（ARGs）在牛粪厌氧消化过程中基于宏基因组学的行为，氧化锌纳米颗粒（ZnO NP）通常用作动物饲料添加剂。总ARGs丰度下降6.6%，而ZnO NP（5 mg/g总固体）的ARGs多样性保持不变，这表明ZnO NP可以通过丰度降低ARGs风险。此外，ZnO NP通过抗生素失活和抗生素靶点改变的机制影响ARGs，并降低潜在宿主（瘤胃杆菌属、Riminococcus属和Paenibacillus属）的丰度，这主要是导致ARGs丰度降低的原因。此外，与没有纳米颗粒的情况相比，使用ZnO纳米颗粒的微生物趋化性降低了17%，这表明潜在宿主受到抑制，他们可以发展适应改变的消化条件的机制，这可能会抑制ARGs的繁殖。这些发现对于促进对含有动物饲料添加剂的牲畜废物处理中ARGs潜在风险的理解非常重要。
Abstract
This work investigated the metagenomics-based behavior of antibiotic resistance genes (ARGs) during cattle manure anaerobic digestion with zinc oxide nanoparticles (ZnO NPs) that are commonly used as animal feed additives. The 6.6% decrease in total ARGs abundance while remained unchanged ARGs diversity with ZnO NPs (5 mg/g total solid), suggested ZnO NPs may mitigate ARGs risk by abundance. Also, ZnO NPs affected ARGs with mechanisms specifically of antibiotic inactivation and antibiotic target change, and declined potential hosts’ abundance (bacterial genus Ruminiclostridium, Riminococcus, and Paenibacillus) which mainly contributed to the decreased ARGs’ abundance. Besides, microbial chemotaxis decreased by 17% with ZnO NPs compared to that without nanoparticles indicated a depression on potential hosts, who could develop the mechanism to adapt to altered digestion conditions, which probably inhibited the ARGs’ propagation. These findings are important to promote understanding of the potential ARGs risks in treatments of livestock wastes containing animal feed additives.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422000384