摘要
      水产养殖沉积物据称是一个相当大的抗生素耐药性基因库。然而，ARGs从沉积物传播给动物和人类的途径尚不清楚。我们在位于中国广东的牛蛙生产设施的沉积物中进行了ARG调查，并构建了模拟斑马鱼繁殖系统，在沉积物中添加或不添加生物炭，以探索生物炭对沉积物和斑马鱼肠道的ARG及其前体的影响。60天后，检测到6种ARGs和intI1亚型，沉积物中的ARGs比斑马鱼肠道中的ARG多。生物炭的添加降低了沉积物和斑马鱼肠道中ARGs的丰度，并抑制了ARGs从沉积物到斑马鱼肠的水平传播。网络分析和偏最小二乘路径模型显示，ARG的富集主要受以氮螺旋菌、Gemmatimonades、Chloroflexi、蓝藻和intI1为主的细菌群的影响。我们的研究结果为ARGs从沉积物向动物的传播提供了见解，并强调了生物炭改良剂对水产养殖沉积物减少ARGs传播的功效。
Abstract
Aquaculture sediments are a purported sizable pool of antibiotic resistance genes (ARGs). However, the pathways for transmission of ARGs from sediments to animals and humans remain unclear. We conducted an ARG survey in sediments from a bullfrog production facility located in Guangdong, China, and simulated zebrafish breeding systems were constructed, with or without biochar addition in sediments, to explore the effects of biochar on ARGs and their precursors of the sediment and zebrafish gut. After 60 days, 6 subtypes of ARGs and intI1 were detected, with sediments harboring more ARGs than zebrafish gut. The addition of biochar reduced the abundance of ARGs in the sediment and zebrafish gut, as well as suppressed the horizontal transmission of ARGs from sediment to zebrafish gut. Network analysis and partial least squares path modeling revealed that ARG enrichment was mainly affected by bacterial groups dominated by Nitrospirae, Gemmatimonades, Chloroflexi, and Cyanobacteria and intI1. Our findings provide insights into the transmission of ARGs from sediment to animals and highlight the efficacy of biochar amendments to aquaculture sediments to reduce the transmission of ARGs.

https://link.springer.com/article/10.1007/s11356-022-19700-2