摘要
      微塑料作为一种新兴的污染物，对环境、动物和人类健康构成潜在风险。MPs在自然生态系统中无处不在，为选择性吸附抗生素抗性基因（ARGs）、细菌（ARB）和细菌组合提供了有利的平台，尤其是在MP、ARGs和ARB热点废水中。在本研究中，研究了不同材料（即聚乙烯、聚氯乙烯和聚对苯二甲酸乙二醇酯）和尺寸（200μm和100μm）的MPs对细胞内ARGs（iARGs）、细胞外ARGs）和细菌组合的选择性捕获。结果表明，iARGs（即i-TetA、i-TetC、i-TetO、i-sul1）、整合素整合酶基因（intI1）和eARGs（如e-TetA和e-laTEM）在MP上选择性富集。在所有MP上，i-sul1、i-TetA和intI1的相对丰度通常高于i-TetC和i-TetO。此外，MPs对废水中微生物群落的形成也有很强的影响，这导致了废水中不同的细菌群落和功能以及对MPs的影响。这些发现表明，MPs可能影响ARB和ARGs在水环境中的选择性富集。
Abstract
Microplastics (MPs), as emerging contaminants, are posing potential risks to environment, and animal and human health. The ubiquitous presence of MPs in natural ecosystems provides favorable platform to selectively adsorb antibiotic resistant genes (ARGs) and bacteria (ARB) and bacterial assemblages, especially in wastewater which is hotspot for MPs, ARGs and ARB. In this study, the selective capture of intracellular ARGs (iARGs), extracellular ARGs (eARGs), and bacterial assemblages by MPs with different materials (i.e. polyethylene, polyvinylchloride, and polyethylene terephthalate) and sizes (200 μm and 100 μm) was investigated. The results showed that iARGs (i.e. i-TetA, i-TetC, i-TetO, i-sul1), integron-integrase gene (intI1), and eARGs (i.e. e-TetA and e-blaTEM) were selectively enriched on MPs. Relative abundances of i-sul1, i-TetA, and intI1 were generally higher than that of i-TetC and i-TetO on all MPs. Moreover, MPs also have strong effects on the formation of microflora in wastewater, which resulted in different bacterial communities and functions in the wastewater and on the MPs. These findings suggested that MPs could affect the selective enrichment of ARB and ARGs in water environment.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722005800