摘要
      目前还没有关于陆地抗生素抗性基因（ARGs）在边缘海域命运的大规模研究报告。选择黄海作为重要的边缘海，研究了边缘海沉积物中目标ARGs的命运、区域分化和驱动因素。ARGs可能从陆地扩散到海洋，海洋/海岸沉积物中总ARGs的平均绝对丰度达到1.23×104/9.79×104拷贝/g。首次观察到黄海冷水团可能对海洋沉积物中ARGs的产生具有潜在的抑制作用。从ARGs、微生物群落和沉积物特征来看，海洋沉积物与海岸沉积物存在显著差异。网络分析表明，ARGs与海岸沉积物中微生物群落的相互作用比海洋沉积物中的相互作用更频繁。人为因素对海洋/海岸沉积物中ARGs的贡献系数为0.524/1.094，而细菌群落对海洋沉积物中ARG的贡献系数主要为0.475，说明陆地ARGs在海洋沉积物中的扩散和增殖可能主要受到人为和微生物因素的影响。这些发现为边缘海中ARGs的命运和驱动因素提供了新的信息。
Abstract
No large-scale investigations on fate of land-based antibiotic resistance genes (ARGs) in marginal sea have been reported. The Yellow Sea which is an important marginal sea was selected to investigate the fate, territorial differentiation, and drivers of target ARGs in marginal-sea sediments. ARGs might spread from land to sea with the average absolute abundance of total ARGs in marine/coastal sediments reaching 1.23 × 104/9.79 × 104 copies/g. The Yellow Sea Cold Water Mass was firstly observed to possibly have potential inhibition effect on occurrence of ARGs in marine sediments. Marine sediments showed significant difference from coastal sediments by considering ARGs, microbial community, and sediment features. Network analysis showed that interaction between ARGs and microbial community in coastal sediments was more frequent than that in marine sediment. The anthropogenic factors posed high positive effect on ARGs in marine/coastal sediments with contribution coefficient of 0.524/1.094 while bacterial community mainly posed positive effect on ARGs in marine sediments with contribution coefficient of 0.475, illustrating that spread and proliferation of land-based ARGs in marine sediments might be mainly affected by anthropogenic and microbial factors. These findings provided new information on fate and drivers of ARGs in marginal sea.

https://www.sciencedirect.com/science/article/abs/pii/S0045653521030125