摘要
      抗生素耐药性基因（ARGs）和毒力因子（VFs）是对人类健康的严重威胁。它们在水生生态系统中的丰度是通过环境外源性物质驱动的选择来维持和增强的。然而，在外源性胁迫下，它们在这些环境中的活性和表达仍然未知。本文检测了在环丙沙星、草甘膦和盐酸舍曲林处理下，ARG和VF在水生微宇宙中的表达谱。环丙沙星增加了ARGs的总表达，尤其是多药耐药基因的表达。在草甘膦和舍曲林处理下，ARGs和VFs的总表达显著降低。然而，在机会性人类病原体中，这些制剂增加了ARGs和VFs的表达。异生物污染物，如我们在这里测试的化合物，有可能破坏微生物生态，促进耐药性，并增加对人类健康的风险。本研究系统地评估了环境外源性物质对ARGs和VFs转录的影响，这两种物质都与人类健康直接相关。这些基因的转录在以前的研究中被忽视了。
Abstract
Antibiotic resistance genes (ARGs) and virulence factors (VFs) are critical threats to human health. Their abundance in aquatic ecosystems is maintained and enhanced via selection driven by environmental xenobiotics. However, their activity and expression in these environments under xenobiotic stress remains unknown. Here ARG and VF expression profiles were examined in aquatic microcosms under ciprofloxacin, glyphosate and sertraline hydrochloride treatment. Ciprofloxacin increased total expression of ARGs, particularly multidrug resistance genes. Total expression of ARGs and VFs decreased significantly under glyphosate and sertraline treatments. However, in opportunistic human pathogens, these agents increased expression of both ARGs and VFs. Xenobiotic pollutants, such as the compounds we tested here, have the potential to disrupt microbial ecology, promote resistance, and increase risk to human health. This study systematically evaluated the effects of environmental xenobiotics on transcription of ARGs and VFs, both of which have direct relevance to human health. Transcription of such genes has been overlooked in previous studies.

https://www.sciencedirect.com/science/article/abs/pii/S0269749122006108