摘要
      在富营养化水体中，磺酰胺类抗生素抗性基因（ARGs），特别是sul1被鉴定为显性基因。小球藻B的表现。研究了地衣群落对sul1的去除、总氮（TN）的去除，以及去除sul1的机理。携带sul1的外源ARGs质粒的去除效率达到（97.2±2.3）%。TN去除率达到（98.5±1.2）%。在藻类和细菌正常生长的前提下，碳代谢、氮代谢、氨酰基tRNA生物合成和糖蛋白的增强对sul1和TN的去除有显著影响。定量聚合酶链反应（qPCR）结果表明，与单个系统（（1×106±15）个基因拷贝/mL）相比，藻类-细菌系统中sul1的绝对丰度较低（0个基因拷贝/mL）。sul1在藻类细胞和细菌细胞中的复制受到抑制。藻类-细菌群落似乎是一种很有前途的废水处理技术，有可能克服富营养化和ARGs的挑战。
Abstract
The sulfonamide antibiotic resistance genes (ARGs) especially sul1 was identified as the dominant in eutrophic water. The performance of Chlorella vulgaris-B. licheniformis consortium toward sul1 removal, total nitrogen (TN) removal, and the mechanism of sul1 removal was investigated. The removal efficiency of exogenous ARGs plasmids carrying sul1 reached (97.2 ± 2.3)%. The TN removal rate reached (98.5 ± 1.2)%. The enhancements of carbon metabolism, nitrogen metabolism, aminoacyl-tRNA biosynthesis, and glycoproteins had significant influences on sul1 and TN removals, under the premise of normal growth of algae and bacteria. The quantitative polymerase chain reaction (qPCR) results suggested that the absolute abundances of sul1 were low in algal-bacterial systems (0 gene copies/mL) compared with individual systems ((1 × 106 ± 15) gene copies/mL). The duplication of sul1 was inhibited in algal cells and bacterial cells. The algal-bacterial consortium seems to be a promising technology for wastewater treatment with a potential to overcome the eutrophication and ARGs challenges.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422012044