摘要
      抗生素耐药性基因（ARGs）的增殖对人类和环境健康构成了重大风险。研究证实，Cu（II）可以加速细菌之间ARGs的偶联转移。这项研究发现，添加溶解的生物炭有效地削弱或消除了Cu（II）促进的ARGs的有效转移。在300°C的热解温度下，用Cu（II）（0.05 mg/L）或溶解的生物炭处理后，偶联转移的效率得到了提高。当暴露于Cu（II）和溶解的生物炭的组合时，转移频率显著降低；这与溶解的生物炭的Cu（II）浓度或热解温度无关。特别地，当Cu（II）浓度超过0.5mg/L时，转移效率被完全抑制。基因表达分析表明，不同的处理通过调节三个全局调控基因的表达来影响转移效率：korA、korB和trbA。其中，腐殖酸抑制了这些基因的表达；然而，Cu（II）与腐殖酸类成分形成络合物，逐渐削弱了这些成分的抑制作用。低分子有机物的促进占主导地位，导致转移效率动态下降。这项研究提供了一种新的环境污染物处理方法，以消除重金属促进的ARGs在细菌之间的转移。
Abstract
The proliferation of antibiotic resistance genes (ARGs) has posed significant risks to human and environmental health. Research has confirmed that Cu(II) could accelerate the conjugative transfer of ARGs between bacteria. This study found that adding dissolved biochar effectively weakened or eliminated the Cu(II)-facilitated efficient transfer of ARGs. The efficiency of conjugative transfer was promoted after treatment with Cu(II) (0.05 mg/L) or dissolved biochar at a pyrolysis temperature of 300 °C. When exposed to the combination of Cu(II) and dissolved biochar, the transfer frequency was significantly reduced; this occurred regardless of the Cu(II) concentration or pyrolysis temperature of dissolved biochar. In particular, when the Cu(II) concentration exceeded 0.5 mg/L, the transfer efficiency was entirely inhibited. Gene expression analysis indicated that different treatments affect transfer efficiency by regulating the expression of three global regulatory genes: korA, korB, and trbA. Among them, humic acid repressed the expression of these genes; however, Cu(II) formed complex with the humic acid-like components, gradually weakening the inhibitive effect of these components. The promotion of low molecule organic matters dominated, resulting in a dynamic decline in the transfer efficiency. This study provides a new environmental contaminant treatment approach to eliminate the heavy metal-facilitated transfer of ARGs between bacteria.

https://www.sciencedirect.com/science/article/abs/pii/S0304389421022196