摘要
      铜绿微囊藻（M.aeruginosa）的存在会影响抗生素抗性基因（ARGs）的转移，而碳基铜纳米复合材料（CCN）的存在则会影响铜绿微囊藻的生长。然而，CCN对铜绿假单胞菌和ARGs的影响尚不完全清楚。在这项研究中，宏基因组测序被用于分析ARGs的可移动性、它们的潜在转移以及在光生物反应器处理城市水中可能的宿主。结果表明，20mg/L的CCN改变了城市水中微生物的组成和丰度，显著促进了铜绿假单胞菌的絮凝，降低了蓝藻和铜绿假单胞杆菌的组成比例。结果表明，20mg/L的CCN显著降低了质粒介导的绝对丰度和ARGs比例（32.7%）。此外，ARGs和可移动遗传元件（MGEs）的低共现概率表明，20mg/L的CCN削弱了由MGEs（如质粒）介导的ARGs的可移动性潜力。在452个宏基因组组装基因组（MAG）中，属于41个细菌类别的95个MAG被鉴定为可能的ARG宿主。这些结果将为城市水中有害蓝藻的控制和ARGs的管理提供见解。
Abstract
The presence of Microcystis aeruginosa (M. aeruginosa) can affect the transference of antibiotic resistance genes (ARGs), and the presence of carbon-based copper nanocomposites (CCN) can affect the growth of M. aeruginosa. However, the effect of CCN on M. aeruginosa and ARGs is not fully understood. In this study, metagenomic sequencing was employed to analyze the movability of ARGs, their potential transfer, and possible hosts in photobioreactor treating urban water. The results uggested that 20 mg/L of CCN changed the composition and abundance of microorganisms in urban water, significantly promoted the flocculation of M aeruginosa, and decreased the composing proportion of Cyanophyta sp. and M aeruginosa. The results indicated that 20 mg/L of CCN significantly decreased the absolute abundance and ARGs proportions which mediated by plasmids (32.7 %). Furthermore, the lower co-occurrence probability of ARGs and mobile genetic elements (MGEs) suggested that 20 mg/L of CCN weakened the movability potential of ARGs mediated by MGEs such as plasmids. Among the 452 metagenome-assembled genomes (MAGs), 95 MAGs belonging to 41 bacterial categories were identified as possible ARG hosts. These results will provide insights into the control of harmful cyanobacteria and the management of ARGs in urban water.

https://www.sciencedirect.com/science/article/abs/pii/S0045653521022165