摘要
      本研究采用强制通风静态堆肥处理猪粪，研究了添加生物炭（BC）和H3PO4改性生物炭（BP）对猪粪堆肥过程中重金属（HMs）稳定性、抗生素抗性基因（ARGs）、重金属抗性基因（MRGs）和细菌群落的影响。堆肥42天后，与对照（CK）相比，BC和BP使二亚乙基三胺五乙酸可提取Cu和Zn的浓度分别降低了12.04%、15.15%和26.91%、36.50%。此外，与对照相比，BC和BP处理使堆肥产品中9种ARG的总丰度分别降低了4.02%和66.21%，5种MRG的总含量分别降低了53.66%和58.81%。网络分析和平方结构方程模型分析表明，BP处理中ARGs和MRGs的减少与堆肥过程中细菌群落的变化有关，而不是与共同选择压力的差异有关。
Abstract
In this research, static composting treatments of swine manure with forced ventilation were conducted to study the effects of biochar (BC) and H3PO4 modified biochar (BP) addition on heavy metals (HMs) stabilization, profiles of antibiotic resistance genes (ARGs), heavy metals resistance genes (MRGs) and bacterial communities during swine manure composting. After 42 days of the composting, compared to control (CK), BC and BP decreased the concentration of diethylenetriamine pentaacetic acid extractable Cu and Zn by 12.04%, 15.15% and 26.91%, 36.50%, respectively. Furthermore, BC and BP treatments reduced the total abundances of nine ARGs by 4.02% and 66.21%, and five MRGs by 53.66% and 58.81%, compared to CK in the compost product. Network analysis and square structural equation model analysis revealed that the decrease of ARGs and MRGs in BP treatment was related to the change in bacterial community during the composting, rather than differences in co-selection pressure.

https://www.sciencedirect.com/science/article/abs/pii/S096085242101974X