摘要
      研究了厌氧消化过程中的恢复性增稠（RT）和热水解（AD）对AD性能、微生物群落和抗生素抗性基因（ARGs）减少的影响。与传统AD（第一阶段）的结果相比，在AD（第三阶段）期间，通过RT（第二阶段），沼气产量略有增加（10%），但通过使用消化物处理系统（DTS，增稠消化物的热水解）的RT，沼气产量增加（63%）。同时，三种不同的操作条件进一步影响了微生物群落结构以及减少ARGs的效率：第二阶段水解菌的丰度和第三阶段产酸菌的丰度增加，第二阶段和第三时期底物中ARGs相对丰度总和的64.3%和47.4%分别被去除。因此，带有DTS的RT-AD可以代表一种有希望改善AD性能的方法，但需要额外的精制以进一步降低消化物中残留ARG的浓度。
Abstract
The effects of recuperative thickening (RT) and thermal hydrolysis during anaerobic digestion (AD) on AD performance, microbial communities and reduction of antibiotic resistance genes (ARGs) were investigated. Compared to the results in conventional AD (Phase I), biogas production increased slightly (10%) through RT (Phase II) but increased (63%) via RT with a digestate treatment system (DTS, thermal hydrolysis of thickened digestate) during AD (Phase III). Meanwhile, the three different operating conditions further influenced microbial community structures as well as efficiencies in reducing ARGs: The abundance of hydrolytic bacteria in Phase II and the abundance of acidogenic bacteria in Phase III increased, and 64.3% and 47.4% of the total sum of the relative abundance of ARGs in the substrate were removed in Phases II and III, respectively. Thus, RT-AD with DTS could represent a promising process for improved AD performance, but requires additional refinements to further reduce concentrations of residual ARGs in digestate.

https://www.sciencedirect.com/science/article/abs/pii/S1385894722036129